Supramolecular Chemistry allows us to understand the interactions that regulate the processes accomplished by living organisms. The intracellular transport of biomacromolecules is of fundamental important in fields such as chemical biology, molecular biology, medicine and beyond. The non-covalent complexation between cationic amphiphiles and anionic nucleic acids and proteins constitutes powerful strategy for gene delivery and gene therapy. We apply the synthetic potential of dynamic covalent bonds (i.e. hydrazone) to tune the properties of polymers and peptides and trigger the delivery of biomacromolecules with biological relevance such as siRNA or DNA and functional proteins including Cas9 for gene edition by CRISPR/Cas9.
We are also interested in applying supramolecular chemistry for the fabrication of artificial tubular networks as cytoskeleton mimics. We use the self-assembly of robust peptide structures in confined spaces constitute as an excellent synthetic tool for the development of bottom up approaches for minimal cell-like entities in synthetic biology.
Conceptual drawing of amphiphilic vehicles and nucleotide cargos for membrane translocation and cell delivery.