The Spectroscopy and Kinetics Unit gives scientific support to the institute’s research groups in spectroscopic techniques and in the study of reaction mechanisms focusing on reaction rates measurements. The unit also collaborates with the research groups in the design and the set up of the kinetic experiments in order to elucidate the reaction mechanism of the systems under study.
The current instrumentation of the unit allows acquisition of UV-Vis, FTIR, FT-Raman, Circular Dichroism and Fluorescence spectra as well as polarimmetric measurements. Moreover, the unit trains the users in order to achieve optimal use of the instruments.
From a mechanistic point of view, the unit tasks involve treatment, fitting and interpretation of the kinetic data. Among other devices, the unit has a stopped flow system to measure fast reaction rates in the millisecond range at different temperatures (from -90 to +100 ºC).
Circular Dichroism Spectrometer
Circular dichroism spectrometer Chirascan from Applied Photophysics, with simultaneous measurement of UV-Vis and CD spectra in the range 165 to 900 nm. The device is equipped with a peltier thermal control unit (-40/+100ºC) with possibility of temperature ramp control.
Fluorescence microwell plate reader
Microplate reader FLUOstar Omega. System composed by a xenon flash-lamp, wheels with 8 excitation and 8 emission filters, temperature control from ambient +5ºC to 45ºC, a reagent injector and a PMT detector. The instrument allows fluorescence and absorbance intensity measurements in the UV/Vis in microplates up to 1536-wells. Wavelength range for fluorescence: 240-740 nm. Wavelength range for absorbance: 220-850 nm.
UV-Vis-NIR spectrophotometer Lambda 1050 from Perkin Elmer. This high performance instrument has double beam optics, double monocromator and three detectors system (PMT, InGaAs and PbS) covering 175 to 3300 nm range. The device is also equipped with a multicell thermostatized 8 positions holder
UV-Vis Spectrophotometer III
Double beam UV-Vis Shimadzu spectrophotometer model UV-1800. Spectral range from 190 to 1100 nm, 1 nm fixed slit.
UV-Vis Spectrophotometer II
Double beam UV-Vis Shimadzu spectrophotometer model UV-1700. Spectral range from 190 to 1100 nm, 1 nm fixed slit.
UV-Vis spectrophotometer I
Double beam UV-Vis Shimadzu spectrophotometer model UV-2401PC with a Peltier thermostated (7-60 ºC) sample holder. Optical range from 190 to 1100 nm, variable slit.
Time Correlated Single Photon Counting (TCSPC) Spectrometer
LifeSpec II from Edinburgh Instruments. This is a compact, high performance, computer controlled spectrometer for the rapid measurement of fluorescence lifetimes based on the technique of Time Correlated Single Photon Counting. The LifeSpec II has two detectors, one standard PMT in the UV-vis range and another PMT nitrogen cooled with spectral response in the vis-NIR region from 300 to 1700nm.
Fast mixing stopped flow module Bio-Logic SFM300, with three syringes controlled independently, that allow simple or double mixing, different mixing ratios and submilisecond mixing time. The system is completed with a cryostat Huber CC3-905 VPCw (-90 ºC to +100 ºC) and a fast UV-Vis Diode Array spectrophotometer TIDAS capable of recording one spectrum in 0.8 ms.
Luminescence Spectrofluorimeter Aminco-Bowman Series 2. System composed by a continuum and a pulsed xenon lamps, four temperature-controlled cuvettes holder (20-60ºC) and a solid sample holder. Wavelength range: 250-850 nm.
Spectrofluorimeter Horiba Jobin Yvon. System composed by a continuum 450W Xenon lamp, double monochromator for excitation, a solid sample holder, detection in Right Angle or Front Face mode and absorbance measurements. The instrument allows fluorescence measurements in the wavelengths range of the UV/Vis and NIR from 250 to 1600 nm.
Portable Raman Spectrometer 785nm
Dispersive Raman Spectrometer model i-Raman from BWTEK. This instrument has an excitation laser at 785nm and a fiber optic probe with a TE Cooled Linear Array detector. The resolution is 3cm-1 and the spectral range goes from 75 to 3200cm-1.
Portable Raman Spectrometer 532nm
Dispersive Raman Spectrometer model i-Raman from BWTEK. This instrument has an excitation laser at 532nm and a fiber optic probe with a TE Cooled Linear Array detector. The resolution is 3cm-1 and the spectral range goes from 75 to 4000cm-1.
Polarimeter Jasco model P-1030 with simmetrical angular oscilation for the Sodium line and photomultiplier tube detector. Angular range: ± 90 º, speed better than 6 º s-1.
FT-IR Vertex 70v Spectrometer from Bruker with vacuum optics and sample compartment. The system has different detectors: DLaTGS, MCT, Bolometer (for Far Infrared measurements) and Photoacustic Cell, covering the range from 8000cm-1 to 10cm-1 with a standard resolution better than 4cm-1. Multiple accessories allow measuring a variety of techniques like Vibrational Circular Dichroism, PMIrras with incidence angles from 32º to 90 º, diffuse reflectance, Rapid Scan and Step Scan.
FTIR spectrometer I
FTIR spectrometer Nicolet is50 from Thermo Scientific, with DLaTGS and MCT detectors, and FTRaman module with the capability of Raman mapping and well plates screening. The instrument also includes a built in ATR module with diamond crystal that allows measuring samples in the Far Infrared (up to 150 cm-1) and Middle Infra-red regions (4000-400cm-1). Spectral range in main sample compartment: 4000–400 cm-1.
FTIR Spectrometer II
FTIR Bruker spectrometer model Alpha with an ATR accessory. Spectral range from 4000 to 400 cm-1.
FTIR Spectometer III
FTIR React IR 15 from Mettler Toledo. The instrument allows in situ reaction monitoring in the mid-infrared. Spectral range: 3000 to 650 cm-1.
FTIR spectrometer IV
FTIR spectrometer Cary 630 from Agilent technologies equipped a DLaTGs detector (4 cm-1 resolution) and a single bounce diamond ATR accessory (spectral range 650-4000cm-1). There is also a DialPath accessory for measuring liquid samples in transmission mode with selectable 30, 50 and 100 μm pathlength.
Microcalorimeter Super CRC 20-250-2.4 from Omnical Technologies to follow heat flow in chemical reactions. Temperature range from -100C to +200C, 1 microwatt sensitivity.